At Nicaea in AD 325 the contested word was homoousios — “of the same substance” as the Father. The rival term was homoiousios, “of like substance.” The two differ by a single iota, and the English saying “not one iota of difference” comes from exactly this.
It makes a fine story and the underlying history is real: the words are real, the parties were real, and the difference between them was not trivial. But the letter did not cause the crisis. It was a marker in an argument about whether the Son is God in the same sense the Father is — an argument that ran for most of a century and would have run without it.
Read this way by: The Nicene controversy; the “iota of difference” proverb is later and English